Table 1.
Primers and probes used in real-time RT-PCR
| Name | Sequence (5′–3′) | Predicted product
|
|
|---|---|---|---|
| Name | Length, bp | ||
| GAPDH f primer | GAA GGT GAA GGT CGG AGT | GAPDH | 155 |
| GAPDH r primer | CAT GGG TGG AAT CAT ATT GGA A | ||
| GAPDH Tq probe | 6FAM-CAA CGG ATT TGG TCG TAT TGG GCG TXTp | ||
| NKA1 f primer | TGA TCA TGG TCC TCC AGG A | NKp30 | 125 |
| NKA1 r primer | AAT GGC CAG TCT CCC TTG G | ||
| NKA1 Tq probe | 6FAM-CCT GTG CTC TCT GGG TGT CCC AGC XTp | ||
| NKG2D f primer | GGC TCC ATT CTC TCA CCC A | NKG2D | 79 |
| NKG2D r primer | TAA AGC TCG AGG CAT AGA GTG C | ||
| NKG2D Tq probe | 6FAM-CCT ACT AAC AAT AAT TGA AAT GCA GAA GGG AGA CTG TXTp | ||
| NKp46 25 f primer | GGC AGA ATC TGA GCG ATG TCT T | NKp46 | 145 |
| NKp46 169 r primer | GCT TTT CCT TTG GAA CCA TGA A | ||
| NKp46 49Tq probe | 6FAM-ACA CTC CCT GCC CTG CTC TGC G TXTp | ||
PCR primers and probes specific for GAPDH, NKA1/NKp30, NKG2D, and NKp46 are listed. Predicted product sizes are indicated on the right. Probes have been labeled with 6-carboxyfluorescein (6FAM) and N,N,N′,N′-tetramethyl-6-carboxyrhodamine (XTp).