Figure 2.

CT60 cell transfected with wild-type NPC1 immunostained for (A) NPC1 protein and (B) lysosomal membrane glycoprotein (lgp95) and (C) stained with filipin for cholesterol localization. (A) NPC1 is present in the transfected cell in granular structures that colocalize with lgp95 (in B at arrows). There are additional lgp95 containing organelles (in B at arrowheads) that do not contain NPC1 protein. Thus NPC1 protein is in a subset of the lgp95-containing organelles. (C) The transfected CT60 cell expressing NPC1 protein does not contain accumulations of intracellular cholesterol in lgp95 containing lysosomes as seen in the four adjacent cells not expressing NPC1 protein. (Bar = 5 μm.)