Table 1.

Correction of the NPC sterol trafficking defect in CT60 cells by expression of NPC1

Transfected plasmid	% Corrected cells
Empty vector	7.9  ±  1.0
Wild-type NPC1	67.8  ±  4.6*
C63S	8.1  ±  1.8
C74S/C75S	5.5  ±  1.0
C97S	8.3  ±  2.9
C-4	7.5  ±  1.0

CT60 cells were transfected with the indicated plasmid and the pEGFP expression plasmid. Cells were incubated with LDL and then fixed for filipin staining as described in the text. One hundred EGFP-stained cells were analyzed for filipin staining in each treatment. The values presented are the percentage of EGFP-expressing cells showing a marked reduction in perinuclear filipin-positive granules. Values are means ±SE from three separate experiments. 

^*Different from all other groups, P < 0.001.