Figure 7.
Stat3 decoy decreases Bcl-xL RNA expression levels compared with mutant control decoy in a SCCHN cell line. Cells from a representative SCCHN cell line (1483) were treated with 25 μM Stat3 decoy in serum-free DMEM. Total RNA was harvested 1 day after treatment. RNase protection assays were then performed. (A) The Bcl-xL and L32 (loading control) bands were quantified by using phosphoimage analysis, and the ratio of Bcl-xL to L32 was calculated for each treatment group. (B) The percent decrease in the ratio of Bcl-xL to L32 in Stat3 decoy-treated cells compared with the mutant control decoy-treated cells was 40% after 24 h of Stat3 decoy treatment.