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. 2003 Mar 24;100(7):4173–4178. doi: 10.1073/pnas.0738063100

Figure 4.

Figure 4

Down-regulation of UNC5H gene expression is associated with allelic loss in UNC5H loci. (A and B) UNC5H allelic loss in colorectal tumors. (A) Fluorescent electropherograms for microsatellite marker D5S1851 on chromosome 5q35.2. The normal (Upper) and tumor (Lower) counterparts of case 14 are shown. a.u., arbitrary units. (B) Summary of LOH analysis for 31 normal/tumor pairs in either UNC5A, UNC5B, or UNC5C locus, showing noninformative (homozygous) markers (white squares), retained markers (shaded squares), and markers with LOH (black squares). Each vertical line indicates a single patient and cases having LOH at one of the UNC5H flanking markers are indicated by *. (C) Effect of trichostatin A and 5aza2dC on UNC5H expression. Northern blot from 5aza2dC-treated (5 μM, 72 h), trichostatin A (TSA)-treated (50 nM, 12 h), or nocodazole (Nocod)-treated (50 ng/ml, 24 h) HT29 cells with an UNC5H2 probe. 28S and 18S RNA are shown as controls for RNA loading.