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Effect of Mg2+ on expression of Csr-regulated genes. Dot blots of total GAS RNA were hybridized to radiolabeled DNA probes specific to the indicated genes, then exposed to x-ray film. GAS wild-type strain 003CAT and the isogenic CsrS mutant strain 003csrSΩ were grown in unsupplemented THY or in THY supplemented with 15 mM MgCl2 (high Mg2+). (A) RNA from GAS collected at exponential phase (A600 = 0.3). (B) RNA collected at stationary phase (A600 = 0.8).
