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. 2003 Mar 20;100(7):4251–4256. doi: 10.1073/pnas.0630496100

Figure 1.

Figure 1

NRG-1 promotes radial glial cell establishment. (a and b) Expression of NRG-1 in WT (a) and NRG−/− (b) E.9.5 telencephalon immunolabeled with anti-NRG1 antibodies. (c–e) Isolated neuroepithelial cells from WT and NRG−/− telencephalic vesicles were cultured at clonal density for 6 days. (c) Clones devoid of RC2-positive cells, clones containing some RC2-positive cells, and clones entirely made of RC2-positive cells were identified. (d) Significant reduction in the number of RC2+ cell-containing clones was noticed in the absence of NRG-1. This deficit was rescued by the addition of exogenous NRG-1. (e) No changes in the generation of neuronal cells were noticed in NRG−/− mice. (f) RT-PCR analysis of Hes1 and neurogenin expression in E9.5 WT and NRG−/− telencephalon. (g) RC2-positive processes of radial glial cells in E9.5 WT telencephalon. (h) Impaired RC2 labeling in littermate NRG−/− telencephalon. Data shown are mean ± SEM; *, P < 0.01, Student's t test. (Scale bar: a, b, g, and h, 10 μm; c, 20 μm.)