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. 2003 Mar 10;100(7):4275–4280. doi: 10.1073/pnas.0530202100

Figure 1.

Figure 1

Generation of fα-CaMKII recombinant mice. (a Top) Region of the wild-type α-CaMKII locus containing the targeted exon 2. (Middle) α-CaMKII targeting vector with a loxP site inserted into intron 1 and an LFNT cassette containing neo-tk and recombinase recognition sites inserted into intron 2. (Bottom) fα-CaMKII locus. Cre/loxP mediated recombination at the fα-CaMKII locus will excise 4 kb of sequence that includes exon 2. Open triangle, loxP; open oval, FRT; neo, neomycin resistance gene; tk, thymidine kinase gene; DT-A, diphtheria toxin gene. Restriction enzymes: B, BamHI; R1, EcoRI; Sp, SpeI; N, NcoI; X, XhoI; Sm, SmaI. (b) Tail DNA digested with BamHI and screened with a 3′ probe external to the recombined locus. +/+, wild type; +/f, heterozygous; f/f, homozygous fα-CaMKII.