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Protease digestion of the free GPI and the procyclin anchor. Wild-type and procyclin null mutant cells were labeled with [3H]ethanolamine and extracted as described in the legends to Figures 4 and 5. The 9% (vol/vol) butan-1-ol extracts of the CMW-soluble (+) fraction containing the free GPI and the CMW-insoluble (−) fraction containing the procyclins were incubated for 24 h in the presence (+) or absence (−) of Pronase and analyzed by SDS-PAGE and autoradiography.
