Figure 8.
Galactose oxidase treatment and sodium [3H]borohydride labeling of living wild-type or null mutant cells. No cell death was observed during the labeling period. Total cell extracts (tot) and the 9% (vol/vol) butan-1-ol extract of the CMW-soluble fraction (CMW) or the CMW-insoluble fraction sequentially extracted with 9% (vol/vol) butan-1-ol (but), 0.1% (wt/vol) Triton X-100 (TX), and 1% (wt/vol) SDS (SDS) were analyzed by SDS-PAGE and autoradiography. Each lane contains equal numbers of cell equivalents from the different fractions.