Figure 5.

The proline-rich domain of Inp53p is required for normal trafficking between the TGN and endosomes. (A) Graphical illustration of wild-type Inp53, HA-Inp53, and HA-Inp53-ΔC proteins. (B) Processing kinetics of A(F→A)-ALP expressed from pSN100 in strains containing the INP53 (SHY35), INP53:: HA (SHY72), and inp53:: HA-CΔ (SHY71) alleles expressing the proteins shown in A. (C) Kinetics of Kex2p turnover in the same strains analyzed in B. The half-times of A(F→A)-ALP processing and Kex2p turnover are indicated below each panel. The experiments shown in B and C were performed as described in the legend to Figures 3 and 4, respectively.