Figure 9.

Combination of inp53 with the gga1Δ gga2Δ mutations causes synthetic phenotypes. (A) Growth of the indicated mutant strains (from top to bottom: SHY35, SHY38, SNY165, SHY60-1A, SHY60-2A, SHY60-6C, UFY2, and SNY173-1B) was assessed by spotting 10-fold serial dilutions onto YEPD plates and growing for 3 d at the indicated temperature. (B) A subset of the strains from (A) carrying plasmid pSN100 to direct expression of A(F→A)-ALP were analyzed for A(F→A)-ALP processing kinetics as described in the legend to Figure 3.