Figure 1.

Identification of proteins complexed with ZW10. Embryo extracts were allowed to bind an anti-ZW10 column, which was washed and eluted as described in MATERIALS AND METHODS. Gels were stained with Coomassie Blue and proteins were identified by MALDI. (A) Total protein before column binding. (B) Elution with 1 M KCl. Cytoplasmic dynein elutes in this fraction (arrow); other bands are nonspecific and occur in fractions from control columns (our unpublished data). (C) Elution with 1.5 M MgCl₂. Proteins identified by mass spectrometry are indicated (arrows). IgG leaching off the column is also prevalent (*).