Skip to main content
. 2003 Apr;14(4):1379–1391. doi: 10.1091/mbc.E02-09-0624

Figure 9.

Figure 9

hROD, hZW10, and hZwilch form a complex in vivo. (A) Specificity of anti-hZwilch. Cell lysates made from GFP-hZwilch–transfected HeLa cells (lanes 1 and 2) and untransfected asynchronous (lane 3) or mitotic (lane 4) HeLa cell lysates were probed with anti-GFP (lane 1) and anti-hZwilch (lanes 2–4). GFP-hZwilch occurs as a 96-kDa protein (arrowhead), whereas hZwilch migrates at ∼75 kDa (arrow). (B) Asynchronous (AS) or mitotic (M) HeLa cell lysates were immunoprecipitated with nonimmune IgG, anti-hZwilch, anti-hROD, or anti-hZW10, and then probed with these antibodies on Western blots as indicated.