Skip to main content
. 2003 Apr;14(4):1392–1404. doi: 10.1091/mbc.E02-03-0156

Figure 7.

Figure 7

Effects of the overexpression of various PDE isoforms on myogenin nuclear accumulation in L6-C5 cells. L6-C5 myoblasts were transiently transfected by different PDE-carrying plasmids or with empty vector (mock) and plated in 10% FBS-containing medium. After 24 h, the medium was replaced by medium containing 1% BSA with or without the addition of 1 nM IGF-I. Myogenin was detected by immunofluorescence, by using the antimyogenin antibody F5D. The results are expressed as percentages of myogenin-positive nuclei (the total number of nuclei was evaluated by UV microscopy using Hoechst 33342 nuclear dye). Normalization of the efficiency of transfection was obtained by cotransfecting a GFP-carrying plasmid. Results are the mean ± SEM of 10 fields counted in a typical experiment. Significantly different from mock-transfected cells cultured without IGF-I: **p < 0.001. Significantly different from mock-transfected cells cultured with IGF-I: +++p < 0.001; ++p < 0.01; +p < 0.05. Overexpression efficiency of the different PDE isoforms was checked by measuring PDE activity in transfected cell homogenates, and normalizing as above. The means ± SEM of 4–5 experiments is shown (inset).