Table 2.

Effect of Tat protein treatment on ⁷⁵Se content of individual selenoproteins

Selenoprotein	75Se content of protein band, arbitrary density units
	Control T cells	Tat-treated T cells
Thioredoxin reductase (57-kDa band)	1,572	1,304
GPX4 (21-kDa band)	975	598
15-kDa proteins (2 bands)	1,766	1,095

The proteins from control T cells and Tat-treated T cells, incubated 50 hr with ⁷⁵Se, are shown in lanes 5 and 10, respectively, of Fig. 4. Aliquots (10 μl) applied to the gel contained 20,000 cpm for the control cell extracat and 47,500 cpm for the Tat-treated cell extract. The arbitrary density units are not directly convertable to cpm ⁷⁵Se.