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Fluorescence signal with selectively permeabilized IRBCs
| Fixation Treatment antisera | no/0.1%
Formaldehyde
|
0.5% Formaldehyde
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| None | SLO | Saponin | T-X-100 | None | SLO | Saponin | T-X-100 | Dried | |
| 10.1 | − | + | + | − | ±a | + | + | − | + |
| 2 | − | + | + | − | ±a | + | + | − | + |
| 4 | − | − | − | − | ±a | + | + | − | + |
| 10.2 | − | − | − | − | ±a | + | + | − | + |
| Aldolase | − | − | − | + | − | ±b | ±b | + | + |
T-X-100, Triton-X-100.
Only few of the IRBCs were labeled.
Very poor signal.
