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. 2003 Apr;14(4):1610–1623. doi: 10.1091/mbc.E02-10-0654

Figure 3.

Figure 3

Vps51p coprecipitates with subunits of the Vps52/53/54 complex. (A) Strains containing chromosomal copies of one or more epitope-tagged proteins (as indicated) were subjected to immunoprecipitation under nondenaturing conditions with rabbit anti-HA antibodies. Copurifying proteins were detected by Western blotting with anti-HA or anti-myc mouse mAbs and species-specific secondary antibodies and visualized by chemiluminescence. Total cell lysates corresponding to 30% of the material used for immunoprecipitation were also analyzed by Western blotting with the same antibodies to confirm the presence of each of the epitope-tagged proteins. The following strains were used: lanes 1 and 6, LCY320; lanes 2 and 7, LCY226; lanes 3 and 8, LCY325; lanes 4 and 9, LCY228; and lanes 5 and 10, LCY227. (B) Lysates were prepared from two different strains, as indicated, and mixed together before immunoprecipitation with anti-HA antibodies as described for A. The following strains were used: lanes 1, 3, 5, and 7, LCY226; lanes 2, 3, 6, and 7, LCY320; lanes 4 and 8, LCY325; and lanes 1, 4, 5, and 8, SNY36–9B.