Figure 2.

hMPS1 is critical for normal mitotic progression. HeLa cells synchronized at the G₁/S boundary were injected with hMPS1 antibodies, fixed 12 and 16 h later, and stained with Cy5 anti-rabbit antibodies to identify the injected cells. Samples at the 12-h time point were also stained with rat anti-MAD1 (a–c) and rat anti-CENP-E (d–f) and counterstained with Alexafluor 488 anti-rat secondary antibodies. At the 16-h time point (g–i), injected cells had divided, as seen by phase-contrast microscopy. DNA was stained with DAPI. The inset shows chromatin bridges or “cut” phenotype in an anaphase cell that had been injected with hMPS1 antibodies. Bar, 10 μm.