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. 2003 Apr;14(4):1664–1676. doi: 10.1091/mbc.E02-09-0602

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Copyright © 2003, The American Society for Cell Biology

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Analysis of rat8-2 rpb1-CTD truncation double mutants. Strains rat8-2 rpb1Δ-187::HIS3 pRPB1 (RPB1/CEN/URA3) and RAT8 rpb1Δ-187::HIS3 pRPB1 were segregants from cross Z26 (rpb1Δ-187::HIS3 pRPB1) × CSY550 (rat8-2). These strains were transformed with plasmids containing RPB1 genes encoding Rpb1p retaining different numbers of CTD repeats: pC3 (10 repeats), pC1 (11 repeats), pC23 (12 repeats), and pV5 (13 repeats). The synthetic interaction was analyzed by spotting serial 1:10 dilution of the transformants on a 5-FOA plate and incubation at 30°C for 3 d.