Figure 5.

Physical interaction between Dbp5p and TFIIH components. Rat8-HA (bottom) from cell extracts was immunoprecipitated with α-HA antibodies (+). Samples with no added primary antibody (−) were used as controls. Total cell extracts (T) contain a 1/200 of the amount of extract used for immunoprecipitation. Samples were analyzed on a 7.5% SDS-PAGE gel. The entire immunoprecipitate was analyzed on the gel. Tfb1p in the immunoprecipitates (IP) was detected with rabbit polyclonal α-Tfb1p and anti-rabbit immunoglobulin G-horseradish peroxidase as the secondary antibody. Genomically myc-tagged Tfb2p and Rad3p were detected with α-myc (mouse) horseradish peroxidase-conjugated antibodies.