Figure 2.

C-terminal rod residues, but not the tailpiece, are conserved in striated muscle myosins. The C-terminal sequences from a diverse array of striated muscle myosins selected from the public database (invertebrate striated, fish and rat skeletal, human and chicken cardiac) are aligned at the beginning of rod zone 40 (McLachlan and Karn, 1982). The essential MHC A region defined by constructs Δ30 and Δ34 (horizontal bar) contains conserved charged residues (arrows) that are likely to be coiled coil (paircoil score p = 1.0; Berger et al., 1995). Boxes indicate serine and threonine residues that might be potential phosphorylation sites. The accession numbers are P12844, M61229, P24733, AAK73348, A24922, A46762, and P29616.