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. 2003 Apr;14(4):1677–1690. doi: 10.1091/mbc.E02-11-0728

Figure 5.

Figure 5

Δ34 protein shows delayed localization into discrete bands during early stages of muscle cell organization. Each column shows embryos from a given strain. Each row depicts a different developmental stage: 1.5-fold (A–D), twofold (E–H), or threefold (I–L). The staining pattern in unc-54(e190) is similar to that in wild-type, despite the lack of MHC B and the resultant reduction in total myosin. The third column shows full-length MHC A that is overexpressed from a transgene in myo-3; stEx80 animals (array chosen for robust rescue; see MATERIALS AND METHODS). Increased MHC A results in stray filamentous staining (arrowhead in G) and occasional aberrant expansion of stain within or near the contractile apparatus (K). However, strong bands of stain are evident at the 1.5-fold stage (C). Distribution of Δ34 protein in myo-3; stEx129 animals is distinct, with diffuse stain outlining the nuclei (D and H). Occasional abnormal round accumulations that are found within the muscle cell body (L) may be due to inappropriate levels of MHC accumulation. Animals are stained with MHC A-specific antibody (5–6), which recognizes endogenous protein in wild-type and unc-54 mutants, and transgenic protein in the remaining genotypes. In wild type, four muscle quadrants run longitudinally along the length of the animal. In most areas, four longitudinal bands of stain are apparent across the width of a single muscle quadrant (between the arrowheads in I). The stages refer to the length of the elongating animal compared with the surrounding eggshell: at 1.5-fold, the posterior tip (arrowhead in A) has reached half the length of the egg as it elongates and moves toward the anterior end (arrow), positioned at the opposite pole of the egg. Bar, 5 μm. Myosin localization during embryogenesis has been described previously (Epstein et al., 1993; Hresko et al., 1994; Williams and Waterston, 1994).