Figure 4.

Developmental phenotypes of rck1 null cells and cells expressing wild-type and mutant RCK1. Cells grown in axenic medium were washed twice with Na⁺/K⁺ phosphate buffer and plated on nonnutrient agar plates. Photographs were taken at various developmental stages. (A) Development phenotypes of rck1 null, RCK1^OE, ^myrRCK1, and RCK1^K867A cells. Wild-type RCK1 and mutant RCK1^K867A were expressed in both wild-type and null backgrounds. (B) Development phenotypes of RCK1 mutants with mutations in the RGS domain in both wild-type and null backgrounds. All photographs were taken at the same magnification..