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Autoradiograms of representative sequencing gels (20% acrylamide/7 M urea). (A) Enzymatic sequencing of 5′-end-labeled E. gracilis U1 snRNA. T1, RNase T1 (cleaves after G); U2, RNase U2 (cleaves after A); M, RNase Phy M (cleaves after A>U); alk, alkali ladder; Nm, O2′-methylnucleoside. (B) Chemical sequencing of the long version (170 nt) of E. gracilis U1 snRNA. Minor bands are the result of cross-contamination with the short version (169 nt) of this RNA species.
