(A) Schematic representation of the secondary structure of HtrI and HtrII. Transmembrane regions (TM1 and TM2), signaling domain (SD), methylation regions (MH1 and MH2), and α5 and α6 [as defined for E. coli Tar and Trg (5, 6, 13)] are labeled. The periplasmic domain of HtrII is drawn as a loop. N, C: N terminus and C terminus of the molecule. (B) Constructs used in this study. For each construct, the encoded Htr and SR proteins are shown. SRI and regions in Htr chimeras that derive from HtrI are not shaded; SRII and regions in Htr chimeras that derive from HtrII are shaded.