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The TOXCAT assay for tm oligomerization. tm-mediated oligomerization of the ToxR cytoplasmic domains (⧫) results in activation of CAT at the ctx promoter by dimerized N-terminal ToxR cytoplasmic domains (■). The periplasmic C-terminal MBP domain (•) anchors the chimera in the inner membrane. ToxR′: the cytoplasmic DNA binding domain of ToxR.
