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Comparison of chimera expression levels in MM39 cells. Anti-MBP immunoblots of serial 2-fold dilutions of cells expressing ToxR′(tm)MBP chimera with wt or mutant (G83I) transmembrane domains or untransformed cells (φ). Arrow indicates full-length chimera. * indicates proteolyzed chimera. For undiluted samples, 4 μl of 6.0 OD600 units of cells were loaded.
