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. 2006 Aug 4;103(33):12569–12574. doi: 10.1073/pnas.0602702103

Fig. 1.

Fig. 1.

Expression of PKD1L3 and PKD2L1 mRNAs in mouse taste tissues. (A) Schematic diagrams of predicted membrane topology of PKD1L3 and PKD2L1. (B) Expression of PKD1L3, PKD2L1, and TRPM5 mRNA in different taste areas. Arrows indicate taste bud regions in the fungiform papillae and the palate. (Scale bar, 50 μm.) (C) PKD1L3 and PKD2L1 are coexpressed in taste cells but not colocalized with TRPM5. Double-label fluorescent in situ hybridization was used to examine PKD1L3, PKD2L1, and TRPM5 expression. The dotted white lines indicate the approximate area of taste buds. (Scale bar, 20 μm.) (D) PKD1L3 and PKD2L1 are specifically expressed in taste tissues. RT-PCR was performed with total RNA from 16 mouse tissues. PCR products were run on agarose gels with markers.