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. 2006 Aug 4;103(33):12569–12574. doi: 10.1073/pnas.0602702103

Fig. 5.

Fig. 5.

Electrophysiological properties of the PKD1L3 and PKD2L1 channel expressed in HEK 293T cells. (A) Representative whole-cell inward currents evoked by 25 mM citric acid (recorded at −60 mV) in cells transfected with PKD1L3 and PKD2L1 cDNAs or vector alone (pCI Vector). Arrows indicate a time point of acid application. (B) A representative whole-cell inward current evoked by 25 mM citric acid in the presence of 100 μM amiloride. (C) Extracellular pH-dependent curves for current activation. Bath solutions containing 5, 10, 15, 20, 25 or 30 mM citric acid (corresponding to pH 3.8, 3.4, 3.0, 2.9, 2.8, or 2.8, respectively) were applied. Currents at each pH were normalized to the currents evoked by application of 25 mM citric acid or HCl at pH 2.5 and averaged across cells (n = 14 for citric acid, n = 9 for HCl). Error bars indicate standard deviation. (D) Current–voltage relationship using a voltage ramp–pulse protocol (−100 to + 40 mV in 100 ms).