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. 2006 Aug 4;103(33):12546–12551. doi: 10.1073/pnas.0605438103

Fig. 1.

Fig. 1.

Genome organization of SARS-CoV recombinant viruses. ORF7a/b of SARS-CoV was replaced with the Renilla luciferase gene, resulting in icSARS-CoV Luc. Second- and third-generation constructs were engineered, which contained two or three mutations in the ORF7a/b TRS, altering the ACGAAC to the double (TRS-1, ACGGAT) or triple (TRS-2, CCGGAT) mutant in icSARS-CoV Luc1 and icSARS-CoV Luc2, respectively. The TRS-2 circuit was placed throughout the icSARS-CoV CRG genome or at select sites within the icSARS-CoV PRG genome to allow for structural gene expression. A series of chimeric viruses was assembled by using the icSARS-CoV and icSARS-CoV CRG molecular clones. (A) Genetic organization of the icSARS-CoV luciferase replacement viruses. Red boxes represent WT virus TRS sites. The TRS-B sites are marked by arrows. (B) Organization of the icSARS-CoV CRG and icSARS-CoV PRG recombinant viruses. TRS sites are indicated by small red (icSARS-CoV) or blue (icSARS-CoV CRG) squares, respectively. (C) Genetic organization of chimeric viruses.