Figure 1.

Characterization of p73β-specific antibody. (A) H1299 cells were transfected with 500 ng of the indicated plasmids. One hundred fifty micrograms of cell extracts prepared 48 hours posttransfection were analyzed by Western blot analysis using the GC15 (left panel) or the ER15 (right panel) antibodies. (B) HCT116(3) cells were either cisplatin-treated (25 or 50 µM) or γ-irradiated (IR: 10 or 20 Gy) and 350 µg of cell extracts prepared 24 hours posttreatment was used for immunoblot analysis using the GC15 and anti-actin antibodies. Extracts from H1299 cells transfected with p73β expression plasmid were used as a positive control. (C) Wild-type and c-Abl null immortalized mouse embryonic fibroblasts were treated with 25 µM cisplatin and analyzed 24 hours after treatment using the GC15 and anti-c-Abl antibodies, as in (B). *Indicates a nonspecific band.