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. 2004 Sep;6(5):584–594. doi: 10.1593/neo.04217

Figure 5.

Figure 5

(A–D) Representative high magnification ERβ immunostaining within the uterine tissue of rat offspring after in utero treatment with EE2 and 0.1 and 50 mg BPA compared with the negative controls. The brown-red color, which indicates ERβ immunoreaction, was recognized dominantly in stromal cells of the mesenchyme (asterisks). (A) Control (cornstarch-treated animals) group. Distinct ERβ immunoreaction in stromal cells of the mesenchyme. Original magnification, x200. (B) 0.2 mg/kg per day EE2 (positive control). Weak immunostained mesenchyme. Original magnification, x200. (C) 0.1 mg/kg per day BPA. Decreased ERβ immunoreactions in stromal cells. Original magnification, x200. (D) 50 mg/kg per day BPA. No ERβ immunoreactions within the uterus. Original magnification, x200. Epithelium (arrow heads).