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. 2004 Sep;6(5):636–645. doi: 10.1593/neo.04229

Figure 5.

Figure 5

Separate evaluation of caspase activation and PARP cleavage in Jurkat.EGP2 target cells and Jurkat bystander cells. Jurkat.EGP2 target cells and Jurkat bystander cells were mixed at target-to-bystander ratio of 1:1 and treated for 6 hours with scFvC54:sTRAIL in the presence or absence of MAb MOC31 or MAb 2E5. After treatment, Jurkat.EGP2 target and Jurkat bystander cells were separated by high-speed cell sorting, after which (A) Jurkat.EGP2 target cells and (B) Jurkat bystander cells were separately analyzed by immunoblot for caspase-8 activation, caspase-3 activation, and PARP degradation. Arrows indicate bands corresponding to cleaved caspase-8. Of note, in the caspase-8 blot of both Jurkat.EGP2 target cells and Jurkat bystander cells, a specific band derived from the heavy chain of MAb MOC31 is also visible.