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. 2004 Sep;6(5):636–645. doi: 10.1593/neo.04229

Figure 6.

Figure 6

Visualization of bystander apoptosis by fluorescence microscopy. U87MG.EGP2 target cells and U87MG bystander cells were mixed at a target-tobystander ratio of 1:4 and precultured on chamber slides. Subsequently, the mixed cultures were subjected for 16 hours to treatment with (A) medium, (B) scFvC54:sTRAIL (300 ng/ml), (C) MAb MOC31 (5 µg/ml) + scFvC54:sTRAIL, and (D) MAb 2E5 (1 µg/ml) + scFvC54:sTRAIL. After treatment, cells were stained using the nuclear stain Hoechst and analyzed for characteristic apoptotic morphology. Target and bystander cells could be distinctly discriminated due to the expression of green fluorescent protein by U87MG.EGP2 target cells only.