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No innocent bystander cell apoptosis in isolated leukocytes. Isolated leukocytes were mixed with Jurkat.EGP2 target cells at a target-tobystander ratio of 1:1. Mixed cultures were then treated for 16 hours with scFvC54:sTRAIL in the presence or absence of MAb MOC31 or MAb 2E5. Apoptosis induction was separately analyzed for leukocytes and Jurkat.EGP2 using PI staining, as described in Materials and Methods section.
