Figure 5.

Effect of geldanamycin on the rate of FAPP secretion by SOJ-6 cells. Subconfluent SOJ-6 cells were incubated in fresh RPMI in the absence (empty column) or presence (dashed column) of 3 µM geldanamycin and further incubated for 6 hours. At the end of incubation, the culture medium was removed. The secretion of FAPP was analyzed by SDS-PAGE and Western blot analysis using pAbL64 (A), and the quantification of this Western blot analysis is performed by the NIH Program (B). The FAPP activity was also determined (C) using 4-nitrophenyl hexanoate as substrate. This experiment was done in triplicate and results are presented as mean ± SD.