Table 2.
Changes in RNA Quality and Gene Expression Ratios Due to the Time Elapsed between Resection and Freezing of Tissue Samples.
| Time from Resection (min) | RNA Quality 28S:18S | Mean Gene Expression Ratio (log2) | SD | Median Gene Expression Ratio (log2) |
| Time course 1 (number of genes = 623) | ||||
| 5 | 2.27 | -0.01 | 0.42 | -0.09 |
| 10 | 2.06 | 0.08 | 0.32 | 0.04 |
| 15 | 2.46 | -0.16 | 0.76 | -0.34 |
| 20 | 2.16 | -0.08 | 0.38 | -0.14 |
| 30 | 2.44 | 0.09 | 0.35 | 0.04 |
| 60 | 2.09 | 0.01 | 0.37 | -0.06 |
| 120 | 2.05 | -0.14 | 0.58 | -0.28 |
| Time course 2 (number of genes = 300) | ||||
| 10 | 1.94 | -0.04 | 0.39 | -0.05 |
| 20 | 2.00 | -0.03 | 0.36 | -0.06 |
| 30 | 1.91 | -0.06 | 0.49 | -0.05 |
| 40 | 2.11 | 0.01 | 0.33 | 0 |
| 50 | 2.03 | -0.15 | 0.46 | -0.16 |
| 60 | 2.56 | -0.32 | 0.47 | -0.41 |