Table 1.

Mutation of Putative E2F Sites in the p73 Promoter.

Mutation	Position	Sequence Change (>AT)
Mut-189	-189 to -182	CTTGGCCC
Mut-155	-155 to -148	CTTCCCGC
Mut-143	-143 to -136	CGGGCTAA
Mut-132	-132 to -125	GGCGCTAA
Mut-6	-7 to +2	CCGCGAAG

All mutations were incorporated using the Quickchange site-directed mutagenesis kit (Stratagene). The putative E2F sites and the nucleotides changed (underlined) are highlighted. An AT dinucleotide was substituted for the underlined nucleotides. The double mutant (Mut-155/-132) incorporates the mutational changes described for Mut-155 and Mut-132.