Table 3.

Regulation of Genes that were not Differentially Following IGF-1 Treatment.

Gene	Regulation	Reference	Expression depends upon
BRCA-1	Cre	[55]	Methylation
Catalase	CCAAT, ERG-1, SP-1, NF-Y	[56]	Acetylation?
Estrogen receptor-alpha	AP-2, CpG islands,	[57–59]	Methylation
Glutathione S-transferase pi	AP-1, SP-1, CpG islands	[60–62]	Methylation
Gycogen synthase kinase-alpha	AP-1, YY-1, LSF, MZF-1, SP-1, CRE	[63]	Unknown
Multidrug resistance-1	AP-1, YB-1, CCAAT	[64,65]	Methylation
Topoisomerase II	CCAAT, Acetylation	[66]	Acetylation
Topoisomerase III	SP-1 (4), YY-1, USF-1	[67]	Methylation

In silico promoter analysis of genes that were printed on the microarray chip but not induced by IGF-1. Eight genes were randomly selected from our gene list and their promoters were queried for transcription factor-binding sites. Although many of the promoters had common transcription factor-binding sites such as CRE and AP-1, their expression depended upon methylation.