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The verification of one- and two-photon colocalization using an in vivo imaging system. WTK1 lymphoblast cells were stained with DiD (A, plasma membrane) and monobromobimane, a GSH-sensitive probe (B, cytoplasm). DiD fluorescence (red) was excited with one photon at 633 nm and monobromobimane fluorescence was excited with two photons at 800 nm. Image (C) shows superimposed images (A) and (B).
