Table 1.
Cell Type | Primer Specificity | Amplified Fragment Size (bp) | Normalized Signal Intensity | Specific Cathepsin B Activity (U/ml cells)* |
GFP-positive | Rat cathepsin B | 162 | 101.8 | 0.015 |
Rat cathepsin H | 529 | 143.5 | ||
Mouse cathepsin B** | 160 | 117.2 | ||
Mouse cathepsin H | 532 | 14.1 | ||
GFP-negative | Rat cathepsin B | 162 | 5.8 | 0.058 |
Rat cathepsin H | 529 | 7.6 | ||
Mouse cathepsin B** | 160 | 354.3 | ||
Mouse cathepsin H | 532 | 44.8 |
Specific activity was determined using a fluorescent substrate as described in Materials and Methods.
Primers specific for mouse cathepsin B are not species-specific.