The NOVH proteins produced by MDCK transfected cells physically interact with fib1C-COOH. Four-microliter samples of conditioned medium (CM) from MDCK-novH sense (S, lane 1) and antisense (AS, lane 2) were incubated with GST–fib1C-COOH and GST–Sepharose beads and further treated as described in the legend of Fig. 2. Lane abbreviations are as in Fig. 2. Lanes 1, 3, 4, and 5: MDCK-novH (S) expressing NOVH. Lanes 2, 6, 7, and 8: control MDCK-novH(AS). ∗ indicates the position of the 48-kDa NOVH protein.