Figure 5.

Selective retention of CTGF onto GST–fib1C-COOH Sepharose beads. Two ml of Sf9/4 conditioned medium were incubated in the presence of either GST–fib1C-COOH (lanes 3–5) or GST (lanes 6–8) Sepharose beads and analyzed as described in Fig. 2. The blots were reacted with the K28T anti-CTGF antibody. Lane 1, untreated conditioned medium from Sf9/4 cells; lane 2, untreated conditioned medium from Sf9/3 cells; other abbreviations are as in Fig. 2.