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. 2003 May;23(9):3226–3236. doi: 10.1128/MCB.23.9.3226-3236.2003

FIG. 4.

FIG. 4.

MBD1 and CAF-1 p150 colocalize to regions of heterochromatin in mouse fibroblasts. NIH 3T3 cells grown on coverslips were cotransfected with the corresponding expression constructs and incubated with anti-Flag MAb and anti-HA polyclonal rabbit primary antibodies. The cells were then washed and incubated with anti-mouse FITC-conjugated and anti-rabbit Cy5-conjugated secondary antibodies. Confocal images of the treated cells were taken to detect FITC (green) and Cy5 (red) immunofluorescence. The two images were merged, and regions of colocalization are indicated by a yellow signal. (A) Coexpressed Flag-CAF-1 p150 and HA-MBD1 colocalize in confocal images of NIH 3T3 cells. (B) Flag-MBD1 colocalizes with HA-HP1α to regions of pericentromeric heterochromatin. (C) Flag-CAF-1 p150 and HA-HP1α colocalize to areas of heterochromatin in mouse cells.