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Modulation between PLD2 and c-Src significantly enhances cellular proliferation. Mouse fibroblasts overexpressing PLD isozymes were plated at a density of 5 × 104 cells in 12-well plates and transfected with empty vector (Vec), wild-type (WT) c-Src, or dominant negative (DN) c-Src mutant. After 40 h of incubation, viable cells were counted by a trypan blue exclusion method. Results show means ± standard deviations of three independent experiments.
