Skip to main content
. 2003 May;23(9):3305–3319. doi: 10.1128/MCB.23.9.3305-3319.2003

FIG. 3.

FIG. 3.

dAda2b, but not dAda2a, associates with dmTaf5, dmTaf9, dmTaf10, dSpt3, and dTra1. Affinity-purified antibodies were conjugated to protein A-Sepharose beads and were used in immunoprecipitation assays of 300 μg of nuclear extract from S2 cells. Input, 30 μg of nuclear extract. (A) Western blots immunoprobed for dSpt3 (left) and dTra1 (right). Lane 1, immunoprecipitates from anti-dAda2a (αdAda2a); lane 2, immunoprecipitates from anti-dAda2b; lane 3, immunoprecipitates from anti-dGcn5; lane 4, immunoprecipitates from anti-dAda3; lane 5, immunoprecipitates from anti-dAda2a; lane 6, immunoprecipitates from anti-dAda2b. (B) Western blots probed for dAda2a (left) and dAda2b (right). Lane 1, immunoprecipitates from anti-dSpt3; lane 2, immunoprecipitates from anti-dAda2b; lane 3, immunoprecipitates from anti-dGcn5; lane 4, immunoprecipitates from anti-dAda3; lane 5, immunoprecipitates from anti-dAda2a; lane 6, immunoprecipitates from anti-dSpt3; lane 7, immunoprecipitates from anti-dGcn5; lane 8, immunoprecipitates from anti-dAda3. hc, IgG heavy chain. (C) Western blots immunolabeled with antibodies against dmTaf5, dmTaf9, and dmTaf10. Lane 1, immunoprecipitates from anti-dAda2b; lane 2, immunoprecipitates from anti-dSpt3; lane 3, immunoprecipitates from anti-dAda3; lane 4, immunoprecipitates from anti-dAda2a.