FIG. 5.

VP16 and Dmp53 interact with dAda2b-containing GNAT complexes. (A) GST pull-down assays were performed with GST-VP16 or GST alone bound to GSH-Sepharose beads and 300 μg of S2 nuclear extract. The precipitates were resolved by SDS-PAGE and transferred onto nitrocellulose membranes for immunodetection assays with antisera against dGcn5, dAda2b, and dAda2a. Input, 30 μg of nuclear extract. (B) GST pull-down assays using a GST-Dmp53 fusion protein. (C) Coimmunoprecipitation assays were performed with anti-Dmp53 antibodies conjugated to protein A-Sepharose and 300 μg of S2 nuclear extract. The immunoprecipitated (IP) proteins were separated by SDS-PAGE prior to Western blotting analyses using antisera against dGcn5, dAda2b, and dAda2a. i, input; be, beads.