FIG. 6.

dAda3 and dGcn5 colocalize on polytene chromosomes from third-instar larvae. Polytene chromosome spreads were double labeled with affinity-purified antibodies against dAda3 (green) and dGcn5 (red). (A) Staining with rabbit anti-dAda3 antibodies. (B) Staining with rat anti-dGcn5 antibodies. (C) Overlap of green and red stains appears in the merge of panels A and B as yellow, yellow-green, and orange. dGcn5 and dAda3 show almost complete overlap. (D) Magnification of the boxed region in panel C. DNA was counterstained with DAPI, which preferentially labels heterochromatin. Note that dAda3 and dGcn5 mainly localize to DAPI-negative euchromatic regions.