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Time-dependent activation of Mxi2. 293T cells transfected with HA-tagged Mxi2, ERK2, and p38 (1 μg) were starved for 12 h and stimulated with 10 μg of anisomycin (aniso) per ml for 20 min or with EGF (100 ng/ml). At the indicated times (minutes), the phosphotransfer activities in the immunoprecipitated kinases were determined by an in vitro kinase assay with MBP as the substrate. c, control.
